SURE-RACE CDNA 5' END DISCOVERY PANELS

Traditional cDNA libraries frequently yield clones which are incomplete and where sequences representing mRNA 5' ends are missing. RACE technology, a PCR-based approach for rapid amplification of cDNA ends, has been particularly useful for completing the missing portions of cDNA clones and yielding full-length sequences of expressed gene regions.

To expand the utility of the RACE technology, OriGene has developed Sure-RACE cDNA 5 End Discovery Panels - PCR-ready RACE panel that allow simultaneous 5' end analysis of transcripts from 24 individual human tissues or 24 mouse tissues and developmental stages. Sure-RACE cDNA 5 End Discovery Panels contain double-stranded cDNAs that have been arrayed in multi-well plates.

• Broad spectrum of tissues: scan 24 individual human or mouse tissues
• High sensitivity: detect rare transcripts
• Simultaneous analysis of alternatively spliced transcripts and alternate RNA start-sites

Sure-RACE Discovery Panels are designed with the recognition that an increasing number of genes have been identified that utilize alternate RNA start-sites resulting from tissue-specific or developmental stage-specific transcriptional promoters. In addition, an increasing number of genes have also been found to utilize alternate 5 exons, sometimes resulting in protein products with different N-terminal amino acid sequences.

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