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Over 1000 citations of OriGene cDNA clones
Bat3 promotes the membrane integration of tail-anchored proteins J. Cell Sci., Jul 2010; 123: 2170 - 2178 [BAT3]
Caveolin-1 modulates HIV-1 envelope induced bystander apoptosis through gp41 J. Virol., Jul 2010; 84: 6515 - 6526 [CAV1]
Chronic lymphocytic leukemia modeled in mouse by targeted miR-29 expression PNAS, Jul 2010; 107: 12210 - 12215 [PXDN]
HIV infection upregulates Caveolin 1 (Cav-1) expression to restrict virus production J. Virol., Jul 2010; 10.1128/JVI.00763-10. [CAV1]
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VCL (NM_003373) Human cDNA ORF Clone

Western Blot Validated

Specifications Related Products Product Manual FAQs
Cat. No. Ref. ID Description Vector Price Delivery
RC204576 NM_003373 Myc-DDK-tagged ORF clone of Homo sapiens vinculin (VCL), transcript variant 2 as transfection-ready DNA pCMV6 Entry
$680
 Immediate
Click here for subcloning services of this insert
into destination vectors		 Please select amount: Add to Shopping Cart

Also for VCL (NM_003373)

OriGene TrueORF Data
Vector: pCMV6 Entry Expression Tag: C-terminal MYC/DDK
Western validation with an anti-DDK antibody
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence 		ORF Size: 3201 bp
Predicted Protein MW: 116.5 kDa
Restriction Sites: Cloning Scheme for this gene
OTI Annotation: This clone expresses the complete ORF with an expression tag.
OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components: The ORF clone is shipped in a 2-D bar-coded Matrix tube as 10 ug transfection-ready dried plasmid DNA.

Reference Data
RefSeq:NM_003373.3, NP_003364
 RefSeq Size: 5443 RefSeq ORF: 3201
Synonyms : CMD1W; CMH15; MVCL
LocusID: 7414 Cytogenetic: 10q22.2 Domains: Vinculin
Summary: Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq].

OriGene offers custom subcloning services to shuttle this insert into any of our destination vectors via PrecisionShuttle for an additional $400 service charge. The delivery takes additional 2 weeks.$1080

Epitope tagging FP tagging Selection markers Inducible Bacterial expression
Destination VectorC-TagN-TagE. coli
Selection		Cell
Selection		ExpressionCustom SubcloningDelivery
pCMV6-AC-HisHis AmpicillinNeomycinMammalian$10802 weeks
pCMV6-AC-MycMyc AmpicillinNeomycinMammalian$10802 weeks
pCMV6-AC-HAHA AmpicillinNeomycinMammalian$10802 weeks
pCMV6-AC-DDKDDK AmpicillinNeomycinMammalian$10802 weeks
pCMV6-AC-Myc-HisMyc-His AmpicillinNeomycinMammalian$10802 weeks
pCMV6-AC-Myc-DDKMyc-DDK AmpicillinNeomycinMammalian$10802 weeks
pCMV6-AC-HA-HisHA-His AmpicillinNeomycinMammalian$10802 weeks
pCMV6-AC-DDK-HisDDK-His AmpicillinNeomycinMammalian$10802 weeks
pCMV6-AN-His HisAmpicillinNeomycinMammalian$10802 weeks
pCMV6-AN-Myc MycAmpicillinNeomycinMammalian$10802 weeks
pCMV6-AN-HA HAAmpicillinNeomycinMammalian$10802 weeks
pCMV6-AN-DDK DDKAmpicillinNeomycinMammalian$10802 weeks
pCMV6-AN-His-Myc His-MycAmpicillinNeomycinMammalian$10802 weeks
pCMV6-AN-Myc-DDK Myc-DDKAmpicillinNeomycinMammalian$10802 weeks
pCMV6-AN-His-HA His-HAAmpicillinNeomycinMammalian$10802 weeks
pCMV6-AN-His-DDK His-DDKAmpicillinNeomycinMammalian$10802 weeks
pCMV6-AC  AmpicillinNeomycinMammalian$10802 weeks
pCMV6-AC-FCFC AmpicillinNeomycinMammalian$10802 weeks
pCMV6-AC-FC-SFC AmpicillinNeomycinMammalian$10802 weeks
pCMV6-AN-FC FCAmpicillinNeomycinMammalian$10802 weeks
pCMV6-AN-FC-S FCAmpicillinNeomycinMammalian$10802 weeks
pCMV6-AC-3DDK3DDK AmpicillinNeomycinMammalian$10802 weeks
pCMV6-AN-3DDK 3DDKAmpicillinNeomycinMammalian$10802 weeks
pCMV6-AN-GFP-C-HisHistGFPAmpicillinNeomycinMammalian$10802 weeks

Peptide sequence of the DDK-tag (Flag®): N-DYKDDDDK-C
Flag® is a registered trademark of Sigma-Aldrich

 
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