TrueORF cDNA Clones: Ready-to-Use Tagged cDNA Clones

TrueORF cDNA clones are tagged cDNA clones for protein studies.

• 25,000 human and 12,000 mouse ORF clones
• 11,000 clones already validated by Western blot
  (see example)
• Two types of ready-to-order clones:
  Myc-DDK tagged or GFP tagged
• 40 destination vectors for additional applications
• Easy shuttling with RapidShuttling kit.
• 10 μg transfection-ready DNA using PowerPrep^® HP Kits

New: Learn about TrueORF and PrecisionShuttle from a 15 min audio slide show.

Search can be performed using: Gene Name, Gene Symbol, Accession Number or Keyword.

Useful links:

TrueORF Entry Vector TrueORF GFP Vector RapidShuttling Kit Destination vectors Product Manual

Related Product:

• TrueClone cDNA clones
• Organelle Marker
• MicroRNA
• VERIFY Tagged Antigen™
• Validated Antibodies for Western Blot
• HuSH-29 shRNA
• MegaTran transfection reagent

Technical features or TrueORF:

• The CMV promoter and a Kozak consensus sequence drive protein expression in mammalian cells.
• The C-terminus of the ORF is tagged with Myc/DDK* or tGFP
• Ten (10) ug of purified plasmid is provided for immediate transfection and expression experiments.
• The upstream T7 promoter allows protein expression in cell-free system.
• Engineered as the entry vector for the OriGene PrecisionShuttle system.

* Peptide sequence of the DDK-tag (Flag®): N-DYKDDDDK-C Flag® is a registered trademark of Sigma-Aldrich