Methyl 2-Cyano-3,12-dioxooleana-1,9-dien-28-oate Decreases Specificity Protein Transcription Factors and Inhibits Pancreatic Tumor Growth: Role of MicroRNA-27aMol. Pharmacol., Aug 2010; 78: 226 - 236
[ZBTB10]
AML1 is overexpressed in patients with myeloproliferative neoplasms and mediates JAK2V617F-independent overexpression of NF-E2Blood, Jul 2010; 116: 254 - 266
[RUNX1]
ORFs cloned in this vector will be expressed in mammalian cells as fusion proteins with 3 tandem DDK-tags on the C-terminal ends, resulting in 100X higher detection sensitivity than 1XDDK tag
The 3DDK tag will increase the affinity of the fusion protein to anti-DDK tags and facilitate detection. This is especially useful for proteins of low expression level.
If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal 3DDK-tagging vector, PS100057.