Coxsackievirus B3 Infection Activates the Unfolded Protein Response and Induces Apoptosis through Downregulation of p58IPK and Activation of CHOP and SREBP1J. Virol., Sep 2010; 84: 8446 - 8459
[ATF6a]
Elevated cyclic AMP (cAMP) levels in T lymphocytes transformed by human T-cell lymphotropic virus type 1 (HTLV-1)J. Virol., Sep 2010; 84: 8732 - 8742
[PDE3B]
ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C- terminal murine Fc tag.
The Fc-tag encodes the CH2 and CH3 domains of the mouse IgG heavy chain and the hinge region. A fusion protein is comprised of two relatively independent parts connected with a flexible spacer. Fc fusion provides several advantages in protein applications:
Easy purification using protein A or protein G affinity chromatography.
Increased solubility.
Excellent antigen in mouse mAB production without cleavage, as the murine Fc is not antigenic in mice.
If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal Fc-tagging vector, PS100055
