ORFs cloned in this vector can be expressed in a tunable manner using various dose of IPTG.
Such clones are the best for genes whose over-expression are toxic to the host cells and those whose expression need to be tightly regulated.
The ORF in this vector is expressed as a tagged protein with the C-terminal DDK-His tags. (DDK is the same as FLAG® which is a registered trademark of Sigma Aldrich). The detection and purification of the transgene expression can be achieved by using anti-Myc or anti-DDK antibodies
