ORFs cloned in this vector will be expressed as a tagged protein with the N-terminal His-DDK tags. (DDK is the same as FLAG® which is a registered trademark of Sigma Aldrich).
Such clones are the best for detection and purification of the transgene expression using anti-DDK or anti-His antibodies or a nickel affinity columns
If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal His-DDK-tagging vector, PS100009
