ORFs cloned in this vector will be expressed as a tagged protein with the C-terminal DDK-His tags. (DDK is the same as FLAG® which is a registered trademark of Sigma Aldrich).
Such clones are the best for detection and purification of the transgene expression using anti-DDK or anti-His antibodies or a nickel affinity columns
If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal DDK-His-tagging vector, PS100018.
