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Over 1000 citations of OriGene cDNA clones
Bat3 promotes the membrane integration of tail-anchored proteins J. Cell Sci., Jul 2010; 123: 2170 - 2178 [BAT3]
Caveolin-1 modulates HIV-1 envelope induced bystander apoptosis through gp41 J. Virol., Jul 2010; 84: 6515 - 6526 [CAV1]
Chronic lymphocytic leukemia modeled in mouse by targeted miR-29 expression PNAS, Jul 2010; 107: 12210 - 12215 [PXDN]
HIV infection upregulates Caveolin 1 (Cav-1) expression to restrict virus production J. Virol., Jul 2010; 10.1128/JVI.00763-10. [CAV1]
View All Citations >>

37,000 TrueORF cDNA clones:
Express proteins with verifiable tags

Seeing is believing, especially when your goal is to achieve protein over-expression.

Engineered as expression-ready clones with verified ORFs and built-in C-terminal tags, TrueORFs express proteins with tracking tags, allowing easy detection and isolation of expressed proteins.  (Learn more..)

ORFWestern

  • 25,000 human and 12,000 mouse clones
  • 5,000 human clones ready for next day delivery
  • Sequence verified
  • Transfection-ready
  • C-terminal Myc-DDK* tag for easy detection
  • Multiple tagging options through PrecisionShuttle
  • Easy search with Gene Name, Gene Symbol, Accession Number or Keyword


All TrueORF inserts are housed in a pCMV6-Entry vector (below) :

 pcmventryvector

Key elements of the TrueORF Entry Vector                                  Download full sequence

Technical features or TrueORF:

  • The CMV promoter and a Kozak consensus sequence drive protein expression in mammalian cells.
  • The C-terminus of the ORF is tagged with both Myc and DDK*
  • Ten (10) ug of purified plasmid is provided for immediate transfection and expression experiments.
  • An antibiotic selection cassette (Neor/Kanr) confers resistance to kanamycin in E. coli and neomycin analogs in mammalian cells.
  • The upstream T7 promoter allows protein expression in cell-free system.
  • Engineered as the entry vector for the OriGene PrecisionShuttle system.

* Peptide sequence of the DDK-tag (Flag®): N-DYKDDDDK-C Flag® is a registered trademark of Sigma-Aldrich
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