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Over 1000 citations of OriGene cDNA clones
Bat3 promotes the membrane integration of tail-anchored proteins J. Cell Sci., Jul 2010; 123: 2170 - 2178 [BAT3]

Caveolin-1 modulates HIV-1 envelope induced bystander apoptosis through gp41 J. Virol., Jul 2010; 84: 6515 - 6526 [CAV1]

Chronic lymphocytic leukemia modeled in mouse by targeted miR-29 expression PNAS, Jul 2010; 107: 12210 - 12215 [PXDN]

HIV infection upregulates Caveolin 1 (Cav-1) expression to restrict virus production J. Virol., Jul 2010; 10.1128/JVI.00763-10. [CAV1]

View All Citations >>

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MicroRNA Expression Plasmid

MicroRNA Processing Diagram

MicroRNAs (miRNAs) are naturally occurring non-coding RNAs of 21-23 nt and they have been implicated in many biological processes and disease development. MiRNA are processed in multiple steps:

DNA→ Pri-miRNA → Pre-miRNA→ Mature miRNA

OriGene provides clones for over-expression of miRNA of your choice. A region of ~600bp region encompassing each miRNA is cloned downstream of a CMV promoter. Upon transfection, the cellular machinery will process the CMV-driven expression of miRNA precursor into mature miRNA and cellular function can be analyzed.

  • Genome wide miRNA coverage (miRBase release 13.0)
    - 646 human and 486 mouse
  • GFP as reporter for transfection monitoring
  • Neomycin selection marker for stable cell establishment
  • 10 μg transfection-ready DNA using PowerPrep® HP Kits
  • TrueClones, cDNA-library sourced clones; most containing 3'-UTRs for miRNA target study
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Catalog No. Description Price
SC4XXXXXX Transfection-ready experssion plasmid for a human or mouse microRNA (10ug) $295

Bulk Order Options

Catalog No. Description Price  
SC410001 Mouse miRNA expression plasmid set (486 vectors, 10ug each in 2-D bar coded tubes) $36,000 shopping cart
SC420001 Human miRNA expression plasmid set (652 vectors, 10ug each in 2-D bar coded tubes) $48,000 shopping cart
SC410002 Mouse miRNA expression plasmid set (486 vectors, 2ug each in 96-well plates) $18,000 shopping cart
SC420002 Human miRNA expression plasmid set (652 vectors, 2ug each in 96-well plates) $24,000 shopping cart

Useful Links

pCMV-MIR Vector Validation Data Product Manual FAQ


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