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MicroRNA Expression Plasmid
MicroRNAs (miRNAs) are naturally occurring non-coding RNAs of 21-23 nt and they have been implicated in many biological processes and disease development. MiRNA are processed in multiple steps:
DNA→ Pri-miRNA → Pre-miRNA→ Mature miRNA
OriGene provides clones for over-expression of miRNA of your choice. A region of ~600bp region encompassing each miRNA is cloned downstream of a CMV promoter. Upon transfection, the cellular machinery will process the CMV-driven expression of miRNA precursor into mature miRNA and cellular function can be analyzed.
- Genome wide miRNA coverage (miRBase release 13.0)
- 646 human and 486 mouse
- GFP as reporter for transfection monitoring
- Neomycin selection marker for stable cell establishment
- 10 μg transfection-ready DNA using PowerPrep® HP Kits
- TrueClones, cDNA-library sourced clones; most containing 3'-UTRs for miRNA target study
| Catalog No. |
Description |
Price |
| SC4XXXXXX |
Transfection-ready experssion plasmid for a human or mouse microRNA (10ug) |
$295 |
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Bulk Order Options
| Catalog No. |
Description |
Price |
|
| SC410001 |
Mouse miRNA expression plasmid set (486 vectors, 10ug each in 2-D bar coded tubes) |
$36,000 |
 |
| SC420001 |
Human miRNA expression plasmid set (652 vectors, 10ug each in 2-D bar coded tubes) |
$48,000 |
 |
| SC410002 |
Mouse miRNA expression plasmid set (486 vectors, 2ug each in 96-well plates) |
$18,000 |
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| SC420002 |
Human miRNA expression plasmid set (652 vectors, 2ug each in 96-well plates) |
$24,000 |
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